Evaluation of synthesized magnetic nanoparticles and salicylic acid effects on improvement of antioxidant properties and essential oils of Calotropis procera hairy roots and seedlings

Calotropis procera (Aiton) W.T.Aiton presents high levels of medicinally important terpenes and phenolics with antiinflammatory, antimicrobial, antioxidant properties. The present study aimed to develop a suitable platform for mass production pharmacological metabolites improvement activity C. procera. Motivated by such aim, potential effects Fe3O4 nanoparticles (Fe3O4 NPs) salicylic acid (SA) on defense response essential oils in seedlings hairy roots were investigated. 21-day-old treated five NPs (0, 50, 100, 150, 200 mg L−1) three SA concentrations (0.0, 0.05, 0.1 mM). Moreover, the about 2 cm transferred into MS liquid medium supplemented above-mentioned 0 0.01 mM SA. After treatment, total phenolics, flavonoids, flavonol, anthocyanin, polyphenol oxidase l-phenylalanine ammonia-lyase, ferric-reducing power, 2, 2‑diphenyl‑1‑picrylhydrazyl analyzed. Results demonstrated that root culture is powerful alternative improving inducing secondary as compared seedlings. it was found combined have more ability than all other treatments improve oil both roots. Here, 100 L−1 had pronounced impact induction constituents activity. suggest can bring great insights development vitro biosynthesis valuable metabolites. Synthesized magnetic nanoparticle may be considered good approach novel